I-25: New Steps towards The Artificial Ovary
Authors
Abstract:
Background: In recent years, transplantation of cryopreserved ovarian tissue has proved to be a promising approach to restore fertility in cancer patients. However, such a procedure cannot be carried out in women at risk of ovarian involvement due to the threat of reintroducing malignant cells. The aim of this study was therefore to create an artificial ovary as an alternative to restore fertility in these patients. Materials and Methods: We tested two fibrin matrices containing low concentrations of fibrinogen (mg/ml) and thrombin (IU/mL) (F/T): F12.5/T1 and F25/T4. Preantral follicles and ovarian (stromal and endothelial) cells (OCs) were isolated from mouse ovaries, encapsulated in the fibrin matrix and autografted to the peritoneal bursa. Results: After one week, the follicle recovery rate ranged between 30.8% (F25/T4) and 31.8% (F12.5/T1). With both fibrin formulations, all follicles were found to be alive or minimally damaged, as demonstrated by TUNEL assay, and at the growing stage (primary, secondary and antral follicles), confirmed by Ki67 immunostaining. Isolated OCs also survived and proliferated after grafting, as evidenced by fewer than 1% of apoptotic cells and a high proportion of Ki67-positive cells. Vessels were found in both fibrin formulations and the global vascular surface area varied from 1.35% (F25/T4) to 1.88% (F12.5/T1). Numerous CD45-positive cells were also observed in F25/T4 and F12.5/T1 combinations. Conclusion: Our results indicate that fibrin is a promising candidate as a matrix for the construction of an artificial ovary. Xenotransplantation of isolated human follicles and OCs is the necessary next step to validate these findings.
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Journal title
volume 8 issue 2.5
pages 10- 10
publication date 2014-07-01
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